Optimum protein function and biochemical activity critically depends on water availability because solvent thermodynamics drive protein folding and macromolecular interactions1. Reciprocally, macromolecules restrict the movement of 'structured' water molecules within their hydration layers, reducing the available 'free' bulk solvent a nd therefore the total thermodynamic potential energy of water, or water potential. Here, within concentrated macromolecular solutions such as the cytosol, we found that modest changes in temperature greatly affect the water potential, and are counteracted by opposing changes in osmotic strength.
"Dr E. Derivery at al."
- October 2023
Understanding the impact of free radicals and antioxidants in cell biology is vital; however, noninvasive nonperturbative imaging of oxidative stress remains a challenge. Here, we evaluated the ability of label-free Raman spectroscopy to monitor redox biochemical changes in antioxidant (N-acetyl-L-cysteine, NAC) and pro-oxidant (tert-butyl hydroperoxide, TBHP) environments. Cellular changes were compared to fluorescence microscopy using CellROX Orange as a marker of oxidative stress.
"Jakub Maciej Surmacki, Isabel Quiros-Gonzalez and Sarah Elizabeth Bohndiek"
- Febuary 2022
Dendritic actin networks grow in an autocatalytic manner starting from the uncapped minifilament of Dynactin.
"Fokin AI, Violaine David, Ksenia Oguievetskaia, Emmanuel Derivery, Caroline E. Stone, Luyan Cao, Nathalie Rocques, Nicolas Molinie, Véronique Henriot, Magali Aumont-Nicaise, Maria-Victoria Hinckelmann, Frédéric Saudou, Christophe Le Clainche, Andrew P. Carter, Guillaume Romet-Lemonne, Alexis M. Gautreau."
- May 2020
Between 6-20% of the cellular proteome is under circadian control to tune cell function with cycles of environmental change. For cell viability, and to maintain volume within narrow limits, the osmotic pressure exerted by changes in the soluble proteome must be compensated.
"Stangherlin A, Wong D, Barbiero S, Watson J, Beale A, Goh C, Feord H, Maywood E, Bartolami E, Matile S, Lequeux N, Pons T, Day J, Van Ooijen G, Derivery E, Edgar R, Newham P & O’Neill J."
- May 2020
"Vincent Rouger, Guillaume Borde, Carole Couillault, Serge Monnere, Sebastien Mailfer, Jonathan J. Ewbank, Nathalie Pujo, Didier Margue"
- May 2014
The Smc5/6 complex is involved in various DNA transactions and is best known for ensuring the fidelity of homologous recombination.
"Thomas J. Etheridge1*, Desiree Villahermosa1, Anja Irmisch1,3, Adam T. Watson1, Alex Herbert1, Hung Q. Dang1,4, Mark A. Osborne2, Antony W. Oliver1, Antony M. Carr1 and Johanne M. Murray"
- June 2020
Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects mitosis.
"Hégarat, N., Crncec, A., Suarez Peredo Rodriguez, M., Echegaray Iturra, F., Gu, Y., Busby, O., Lang, P., Barr, A., Bakal, C., Kanemaki, M., Lamond, A., Novak, B., Ly, T. and Hochegger, H., 2020. Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. The EMBO Journal, 39(11)."
- March 2020
"Tom Stiff, Fabio R. Echegaray-Iturra, Harry J. Pink, Alex Herbert, Constantino Carlos Reyes-Aldasoro, Helfrid Hochegger."
- May 2020